IgG Food MAP with Candida
Includes a wide range of foods, common in the Western, Asian, and Mediterranean diets. We have added a hemp allergy marker because it is very common now as a food source or as medical CBD. We are able to run the IgG Food MAP on both serum and dried blood spot samples
ASSESSES 190 FOODS
Includes a wide range of foods, common in the Western, Asian, and Mediterranean diets. We have added a hemp allergy marker because it is very common now as a food source or as medical CBD.
SUPERIOR SENSITIVITY AND ACCURACY COMPARED TO ELISA
GPL’s xMAP® immunoassay with fluorescence readout has proven to be even more sensitive than ELISA tests. The IgG Food MAP test detects all food antibodies with greater precision than ELISA tests thanks to the superior technology we’ve developed for the test.
Antigen-specific IgG antibodies are captured on multiplexed magnetic beads, using xMAP® (Multiple Analyte Profiling) technology. The xMAP® bead-based immunoassay allows for the simultaneous detection of IgG antibodies to all 190 foods in two single test wells, reducing sample volume requirements and reagents while increasing sensitivity and specificity as compared to traditional plate-based ELISA tests. The upgraded immunoassay is cost and time effective, easy to perform and reproducible. We have now fully automated all steps of our testing processes, leading to even better precision.
The xMAP® bead-based immunoassay is environmentally friendly, reducing plastic pollution. In the ELISA, a panel for 190 foods would require 190 wells (2 plates) per sample. In contrast, the beadbased platform only requires two wells per sample. In other words, the bead-based immunoassay would use just one plastic microtiter plate for testing 48 samples, while the ELISA would use 96 plates to test the same number of samples.
GREATER RELIABILITY OF RESULTS
In the multiplexed bead-based immunoassay, food antigenic proteins are first covalently bound to beads. The beads are mixed with the patient’s sample where bead-bound antigens capture the specific IgG antibodies present in the sample. The food-specific IgG antibody level of each sample is detected with a fluorescent-labeled antibody against IgG. Every reaction with a patient sample also contains control beads to ensure accurate results. The fluorescent signal is measured using an xMAP® analyzer. The amount of food-specific antibody detected per allergen specific bead is directly proportional to the fluorescent signals. The higher the fluorescence, the more specific IgG antibodies are present in the sample. Once test results are available for review, our Quality Assurance (QA) team carefully verifies that quality control measures are within instrument and assay specifications.
TESTS FOR SPECIFIC FOOD ANTIGENIC PROTEINS
detection to multiple analyte detection. The multiplexed, bead-based assay only detects IgGs against food antigenic proteins that were covalently bound to the beads, making this assay more specific to proteins than the ELISA. In the ELISA, there is no covalent binding of proteins; instead, proteins or other antigens are bound to the surface of a plastic well by adsorption. This non-specificity of the surface allows the binding of glycan-rich molecules such as Candida and yeast. Because of this, GPL is providing a separate report for the yeasts which will be tested by ELISA, rather than the xMAP® immunoassay.